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INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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The most potent isolate was identified as Streptomyces grisenus. Elution was carried out by 0.

Elsevier Applied Science, London, pp: The results in Table 6 and 7 revealed the effect of some physical conditions on purified enzyme produced by streptomyces grisenus viz.

Beta fructofuranosidases of Helianthus inulinade L. The analysis of the deduced inulinase amino acid sequence revealed the presence of a putative gram-positive signal peptide and a cleavage site following amino acid 39 was predicted with the program designed by Nielsen et al.

Production of inulin fructotransferase depolymerizing by Artrobacter sp. After separation, the proteins were transferred to a polyvinylidene difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid inukinase analysis.

Characleristics of an inulinase inuinase by bacillus subtilis A, astrain isolated from the rhizosphere of Ueronia herbacea velly rusby. As a result of reaction, reducing sugar was determined by the 3. Close Print this page. Present findings are in agreement with Lim et al. Co-operative description of type strain of Streptomyces.

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Production, Purification, Characterization and Applications of Fungal Inulinases

Optimisation of inulinase production by Kluyveromyces bulgaricus. As shown in Table 1 the maximum activity was found on the 2nd day of incubation period.

Abstract Streptomyces grisenus isolated from soil, was found to produce a very active inulinase enzyme. Then, the enzyme precipitate was dissolved in 0.

Its production, properties and industrial applications. The bacterial culture used in this study was the strongest inulinalytic strain among 50 isolates: Hydrolysis of inulin from jerusalem artichake by inulinase immobilized on aminoethyl cellulose enzyme.

Use of dinitrosalicylic acid reagent for determination of reducing sugar. Purification and Characterization of Exoinulinase Enzyme from Streptomyces grisenus.

Inulinase activity was assayed in the reaction mixture inoculated with purifocation enzyme concentration 0. Increased bioavailability of minerals, lowering of serum lipids levels relevant for cardiovascular disease and stimulation immune response Coudray et al.

It was identified and confirmed according to description of ISP reported by Shirling and Gottliebaband and key of Bergey’s Manual of Williams et al. The metabolism of fructose polymers in plants.

Additional species description from first and second studies. Effect of carbon source on the inulinase activity of Streptomyces grisenus. Inulin can be considered as dietary fibre, a substitute for fat and low calorie sweetener Roberfroid, Effect of incubation period on the inulinase activity of Streptomyces grisenus. However pugification enzyme was stable in the presence of CaCl 2.

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Considerable evidences shows that inulinases are important industrial enzymes; however, the information on microbial sources, characteristics, and applications of inulinases is scattered in the available literature.

Highly active isolate of Streptomyces grisenus isolated from soil sample and identified according to Cause et al. Assay of inulinase activity and protein content of each fraction were examined.

Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

Utilization of chicory roots for microbial endoinulinase production. Methods for characterization of Streptomyces species. Gel filtration using sephadex G Besides, various promising applications and aspects of enzymatic hydrolysis for producing important industrial products also have been discussed.

Recent developments in microbial inulinases. Dietary fiber, inulin and oligofructose: The results in Table 2 revealed that the maximum activity was observed at pH 7.

Inulijase can be found in plants and microorganisms. The precipitate formed was obtained by centrifugation in an ultracentrifuge at The production broth medium was optimized for maximum inulinase production by addition of 0.